Family-wide Structural and Biophysical Analysis of Binding Interactions among Non-clustered δ-Protocadherins.

ABSTRACT: Non-clustered δ1- and δ2-protocadherins, close relatives of clustered protocadherins, function in cell adhesion and motility and play essential roles in neural patterning. To understand the molecular interactions underlying these functions, we used solution biophysics to characterize binding of δ1- and δ2-protocadherins, determined crystal structures of ectodomain complexes from each family, and assessed ectodomain assembly in reconstituted intermembrane junctions by cryoelectron tomography (cryo-ET). Homophilic trans (cell-cell) interactions were preferred for all δ-protocadherins, with additional weaker heterophilic interactions observed exclusively within each subfamily. As expected, δ1- and δ2-protocadherin trans dimers formed through antiparallel EC1-EC4 interfaces, like clustered pro

SUBMITTER: Harrison OJ 

PROVIDER: S-EPMC7082078 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature