Nanopore analysis: An emerging technique for studying the folding and misfolding of proteins.

ABSTRACT: Nanopore analysis is an emerging technique that enables the investigation of the conformation of a single peptide or protein molecule. Briefly, a pore is inserted into a membrane under voltage clamp conditions. When a molecule interacts with the pore there is a change in the current, I, for a time, T. Small unfolded molecules can translocate the pore whereas folded or large molecules tend to simply bump into the pore and then diffuse away. Therefore, the parameters, I and T, are dependent on the conformation of the molecule at the instant at which it encounters the pore. Thus, multiple conformations can be detected simultaneously in a single sample. As well, the analysis can be performed under dilute conditions so that folding or dimerization of a peptide can be followed in real time, whic

SUBMITTER: Madampage C 

PROVIDER: S-EPMC7082088 | biostudies-literature | 2012 Apr-Jun

REPOSITORIES: biostudies-literature