N⁶-methyladenosine mediates arsenite-induced human keratinocyte transformation by suppressing p53 activation.

ABSTRACT: N⁶-methyladenosine (m⁶A), the most abundant and reversible RNA modification, plays critical a role in tumorigenesis. However, whether m⁶A can regulate p53, a leading antitumor protein remains poorly understood. In this study, we explored the regulatory role of m⁶A on p53 activation using an arsenite-transformed keratinocyte model, the HaCaT-T cell line. We created the cell line by exposing human keratinocyte HaCaT cells to 1 μM arsenite for 5 months. We found that the cells exhibited an increased m⁶A level along with an aberrant expression of the methyltransferases, demethylase, and readers of m⁶A. Moreover, the cells exhibited decreased p53 activity and reduced p53 phosphorylation, acetylation, and transactivation with a hi

SUBMITTER: Zhao T 

PROVIDER: S-EPMC7082205 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature