Novel syntrophic bacteria in full-scale anaerobic digesters revealed by genome-centric metatranscriptomics.

ABSTRACT: Short-chain fatty acid (SCFA) degradation is an important process in methanogenic ecosystems, and is usually catalyzed by SCFA-oxidizing bacteria in syntrophy with methanogens. Current knowledge of this functional guild is mainly based on isolates or enrichment cultures, but these may not reflect the true diversity and in situ activities of the syntrophs predominating in full-scale systems. Here we obtained 182 medium to high quality metagenome-assembled genomes (MAGs) from the microbiome of two full-scale anaerobic digesters. The transcriptomic response of individual MAG was studied after stimulation with low concentrations of acetate, propionate, or butyrate, separately. The most pronounced response to butyrate was observed for two MAGs of the recently described genus Candidatus Phosphit

SUBMITTER: Hao L 

PROVIDER: S-EPMC7082340 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature