New Insights Into <i>Acidithiobacillus thiooxidans</i> Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses.

ABSTRACT: Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S⁰ and S2⁢O32- metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectroscopy. The A. thiooxidans S2⁢O32- metabolism model involves the conversion of S2⁢O32- to SO42- , S⁰ and S4⁢O62-, mediated by the sulfur oxidase complex (Sox), tetrathionate hydrolase (TetH), sulfide quinone reductase (Sqr), and heterodisulfate reductase (Hdr) proteins. These same proteins, with the addition of rhodanese (Rhd), were identified to convert S⁰ to SO32- , S2⁢O32- and polythionates in the A. thiooxidans S⁰ metabolism model. Our combine

SUBMITTER: Camacho D 

PROVIDER: S-EPMC7082400 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature