A high-throughput method for dereplication and assessment of metabolite distribution in Salvia species using LC-MS/MS.

ABSTRACT: Dereplication of crude plant extracts through liquid chromatography-mass spectrometry is a powerful technique for the discovery of novel natural products. Unfortunately, this technique is often plagued by a low level of confidence in natural product identification. This is mainly due to the lack of extensive chromatographic and mass spectrometric optimizations that result in improper and incomplete MS/MS fragmentation data. This study proposes a solution to this problem by the optimization of chromatographic separation and mass spectrometry parameters. We report herein a direct and high-throughput strategy for natural product dereplication in five Salvia species using high-resolution ESI-QTOF-MS/MS data. In the present study, we were able to identify a total of forty-seven natural p

SUBMITTER: Ul Haq F 

PROVIDER: S-EPMC7082496 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature