Beta cell identity changes with mild hyperglycemia: Implications for function, growth, and vulnerability.

ABSTRACT:

Objective

As diabetes develops, marked reductions of insulin secretion are associated with very modest elevations of glucose. We wondered if these glucose changes disrupt beta cell differentiation enough to account for the altered function.

Methods

Rats were subjected to 90% partial pancreatectomies and those with only mild glucose elevations 4 weeks or 10 weeks after surgery had major alterations of gene expression in their islets as determined by RNAseq.

Results

Changes associated with glucose toxicity demonstrated that many of the critical genes responsible for insulin secretion were downregulated while the expression of normally suppressed genes increased. Also, there were marked changes in genes associated with replication, aging, senescence, stress, inflammation

SUBMITTER: Ebrahimi AG 

PROVIDER: S-EPMC7082551 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature