Two New Unspecific Peroxygenases from Heterologous Expression of Fungal Genes in Escherichia coli.

ABSTRACT: Unspecific peroxygenases (UPOs) constitute a new family of fungal heme-thiolate enzymes in which there is high biotechnological interest. Although several thousand genes encoding hypothetical UPO-type proteins have been identified in sequenced fungal genomes and other databases, only a few UPO enzymes have been experimentally characterized to date. Therefore, gene screening and heterologous expression from genetic databases are a priority in the search for ad hoc UPOs for oxyfunctionalization reactions of interest. Very recently, Escherichia coli production of a previously described basidiomycete UPO (as a soluble and active enzyme) has been reported. Here, we explored this convenient heterologous expression system to obtain the protein products from available putative UPO ge

SUBMITTER: Linde D 

PROVIDER: S-EPMC7082571 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature