Toward Automatic and Comprehensive Glycan Characterization by Online PGC-LC-EED MS/MS.

ABSTRACT: Despite the recent advances in mass spectrometry (MS)-based methods for glycan structural analysis, characterization of glycomes remains a significant analytical challenge, in part due to the widespread presence of isomeric structures and the need to define the many structural variables for each glycan. Interpretation of the complex tandem mass spectra of glycans is often laborious and requires substantial expertise. Broad adoption of MS methods for glycomics, within and outside the glycoscience community, has been hindered by the shortage of bioinformatics tools for rapid and accurate glycan sequencing. Here, we developed an online porous graphitic carbon liquid chromatography (PGC-LC)-electronic excitation dissociation (EED) MS/MS method that takes advantage of the superior isomer resolv

SUBMITTER: Wei J 

PROVIDER: S-EPMC7082718 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature