Low-Resource Nucleic Acid Extraction Method Enabled by High-Gradient Magnetic Separation.

ABSTRACT: Nucleic acid-based diagnostic tests often require isolation and concentration of nucleic acids from biological samples. Commercial purification kits are difficult to use in low-resource settings because of their cost and insufficient laboratory infrastructure. Several recent approaches based on the use of magnetic beads offer a potential solution but remain limited to small volume samples. We have developed a simple and low-cost nucleic acid extraction method suitable for isolation and concentration of nucleic acids from small or large sample volumes. The method uses magnetic beads, a transfer pipette, steel wool, and an external magnet to implement high-gradient magnetic separation (HGMS) to retain nucleic acid-magnetic bead complexes within the device's steel wool matrix for subsequent p

SUBMITTER: Pearlman SI 

PROVIDER: S-EPMC7082792 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature