High frequency optogenetic activation of inputs to the lateral amygdala forms distant association with foot-shock.

ABSTRACT:

Aim

A hallmark of classical conditioning is that conditioned stimulus (CS) must be tightly coupled with unconditioned stimulus (US), often requiring temporal overlap between the two, or a short gap of several seconds. In this study, we investigate the temporal requirements for fear conditioning association between a strong artificial CS, high-frequency optogenetic activation of inputs into the lateral amygdala of rats, and a foot-shock to the animal with delays up to many minutes.

Methods

AAV-oChIEF-tdTomato viruses were injected into the auditory cortex and the medial geniculate nucleus of rats. An optical fiber was implanted just above the lateral amygdala of the animal. Optogenetic high-frequency stimuli (oHFS; containing five 1-s trains of 100 Hz laser pulses) were deliv

SUBMITTER: Li F 

PROVIDER: S-EPMC7082919 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature