Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis.

ABSTRACT:

Background

Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterization of sRNAs in plants typically relies on the modification of the steady state levels of these molecules. State-of-the-art strategies to reduce plant sRNA levels include molecular tools such as Target Mimics (MIMs or TMs), Short Tandem Target Mimic (STTMs), or molecular SPONGES (SPs). Construction of these tools routinely involve many different molecular biology techniques, steps, and reagents rendering such processes expensive, time consuming, and difficult to implement, particularly high-throughput approaches.

Results

We have developed a vector and a cloning strategy

SUBMITTER: Lopez-Marquez D 

PROVIDER: S-EPMC7082952 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature