Hypoxia-preconditioned mesenchymal stem cells prevent renal fibrosis and inflammation in ischemia-reperfusion rats.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) have been reported to promote the regeneration of injured tissue via their paracrine abilities, which are enhanced by hypoxic preconditioning. In this study, we examined the therapeutic efficacy of hypoxia-preconditioned MSCs on renal fibrosis and inflammation in rats with ischemia-reperfusion injury (IRI).

Methods

MSCs derived from rats and humans were incubated in 1% O₂ conditions (1%O₂ MSCs) for 24 h. After IRI, 1%O₂ MSCs or MSCs cultured under normoxic conditions (21%O₂ MSCs) were injected through the abdominal aorta. At 7 or 21 days post-injection, the rats were sacrificed and their kidneys were analyzed. In in vitro experiments, we examined whether 1%O₂ MSCs enhanced the abi

SUBMITTER: Ishiuchi N 

PROVIDER: S-EPMC7083035 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature