Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells.

ABSTRACT:

Background

Organ replacement regenerative therapy based on human adult stem cells may be effective for salivary gland hypofunction. However, the generated tissues are immature because the signaling factors that induce the differentiation of human salivary gland stem cells into salivary glands are unknown.

Methods

Isolated human submandibular gland stem/progenitor cells (hSMGepiS/PCs) were characterized and three-dimensionally (3D) cultured to generate organoids and further induced by fibroblast growth factor 10 (FGF10) in vitro. The induced spheres alone or in combination with embryonic day 12.5 (E12.5) mouse salivary gland mesenchyme were transplanted into the renal capsules of nude mice to assess their development in vivo. Immunofluorescence, quantitative reverse transcrip

SUBMITTER: Sui Y 

PROVIDER: S-EPMC7083056 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature