Chemical-defined medium supporting the expansion of human mesenchymal stem cells.

ABSTRACT:

Objectives

Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet.

Materials and methods

Here, we developed the full chemical-defined medium, NBVbe medium, via RNA sequencing, bioinformatic analysis, and growth factor screening.

Results

The NBVbe medium contains N2B27 medium with the BSA (bovine serum albumin) replaced by the recombinant human albumin, bFGF (basic fibroblast growth factor), vitamin C, and EGF (epidermal growth factor). The NBVbe medium could support the isolation and expansion of human MSCs from the umbilical cords.

Conclusions

The full chemical-defined medium supporting the isol

SUBMITTER: Xu J 

PROVIDER: S-EPMC7083066 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature