The Degree of t-System Remodeling Predicts Negative Force-Frequency Relationship and Prolonged Relaxation Time in Failing Human Myocardium.

ABSTRACT: The normally positive cardiac force-frequency relationship (FFR) becomes flat or negative in chronic heart failure (HF). Here we explored if remodeling of the cardiomyocyte transverse tubular system (t-system) is associated with alterations in FFR and contractile kinetics in failing human myocardium. Left-ventricular myocardial slices from 13 failing human hearts were mounted into a biomimetic culture setup. Maximum twitch force (F), 90% contraction duration (CD₉₀), time to peak force (TTP) and time to relaxation (TTR) were determined at 37°C and 0.2-2 Hz pacing frequency. F_{1 Hz}/F₀._{5 Hz} and F_{2 Hz}/F₀._{5 Hz} served as measures of FFR, intracellular cardiomyocyte t-tubule dis

SUBMITTER: Abu-Khousa M 

PROVIDER: S-EPMC7083140 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature