Overexpression of Myo1e promotes albumin endocytosis by mouse glomerular podocytes mediated by Dynamin.

ABSTRACT:

Background

As a fundamental process internalizing molecules from the plasma membrane, endocytosis plays a crucial role in podocyte biology. Our previous study has identified that overexpression of Myole may enhance podocyte endocytosis. However, its potential mechanism has been not well understand. Thus, we aimed to analyze whether albumin endocytosis by mouse glomerular podocytes is dependent on Myo1e expression. Also, we aimed to elucidate whether the underlying mechanism is mediated by Dynamin.

Methods

Firstly, mouse podocyte cells (MPC5) were treated with different concentrations of FITC-bovine serum albumin (BSA). The fluorescence intensity and cell viability were detected by flow cytometry and MTT assays, respectively. Afterwards, the optimal concentration of FITC-BSA

SUBMITTER: Shen H 

PROVIDER: S-EPMC7083160 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature