Toll-like receptor 2 stimulation augments esophageal barrier integrity.

ABSTRACT:

Background

Germline-encoded innate immune pattern recognition receptors (PRR) are expressed at epithelial surfaces and modulate epithelial defenses. Evidence suggests that stimulation of the Toll-like receptor (TLR) family of PRR may regulate epithelial barrier integrity by upregulating tight junction (TJ) complex protein expression, but it is not known whether this mechanism is utilized in esophageal epithelial cells. TJ complex proteins maintain intact barrier function and are dysregulated in atopic disorders including eosinophilic esophagitis.

Methods

Pattern recognition receptors expression was assessed in EoE and control primary esophageal epithelial cells, demonstrating robust expression of TLR2 and TLR3. The three-dimensional air-liquid interface culture (ALI) model w

SUBMITTER: Ruffner MA 

PROVIDER: S-EPMC7083217 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature