Targeted Sequencing of Genomic Repeat Regions Detects Circulating Cell-free Echinococcus DNA.

ABSTRACT: BACKGROUND:Echinococcosis is a chronic zoonosis caused by tapeworms of the genus Echinococcus. Treatment of the disease is often expensive and complicated, sometimes requiring extensive surgery. Ultrasonographic imaging is currently the main technique for diagnosis, while immunological analysis provides additional information. Confirmation still needs pathological analysis. However, these diagnostic techniques generally detect infection in late stages of the disease. An accurate, early and non-invasive molecular diagnostic method is still unavailable. METHODOLOGY/PRINCIPAL FINDINGS:We sequenced the cell-free DNA (cfDNA) from plasma of echinococcosis patients and confirmed the presence of Echinococcus DNA. To improve detection sensitivity, we developed a method based on targeted next-genera

SUBMITTER: Wan Z 

PROVIDER: S-EPMC7083330 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature