High-throughput 16S rDNA sequencing of the pulmonary microbiome of rats with allergic asthma.

ABSTRACT: A decrease in microbial infection in adolescents is implicated with an increase in the incidence of asthma and allergic diseases in adulthood, indicating that the microbiome plays a critical role in asthma. However, the microbial composition of the lower respiratory tract remains unclear, hindering the further exploration of the pathogenesis of asthma. This study aims to explore the microbial distribution and composition in the lungs of normal rats and rats with allergic asthma via 16S rDNA sequencing. The DNA of the pulmonary microbiome was extracted from the left lungs collected from normal control group (NC), saline control group (SC), and allergic asthma group (AA) under aseptic conditions. After the 16s rDNA V4-V5 region was amplified, the products were sequenced using Illumina high-t

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC7083718 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature