Benchmarking the MinION: Evaluating long reads for microbial profiling.

ABSTRACT: Nanopore based DNA-sequencing delivers long reads, thereby simplifying the decipherment of bacterial communities. Since its commercial appearance, this technology has been assigned several attributes, such as its error proneness, comparatively low cost, ease-of-use, and, most notably, aforementioned long reads. The technology as a whole is under continued development. As such, benchmarks are required to conceive, test and improve analysis protocols, including those related to the understanding of the composition of microbial communities. Here we present a dataset composed of twelve different prokaryotic species split into four samples differing by nucleic acid quantification technique to assess the specificity and sensitivity of the MinION nanopore sequencer in a blind study design. Taxono

SUBMITTER: Leidenfrost RM 

PROVIDER: S-EPMC7083898 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature