Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism.

ABSTRACT: Dysregulated cholesterol metabolism is implicated in a number of neurological disorders. Many sterols, including cholesterol and its precursors and metabolites, are biologically active and important for proper brain function. However, spatial cholesterol metabolism in brain and the resulting sterol distributions are poorly defined. To better understand cholesterol metabolism in situ across the complex functional regions of brain, we have developed on-tissue enzyme-assisted derivatization in combination with microliquid extraction for surface analysis and liquid chromatography-mass spectrometry to locate sterols in tissue slices (10 µm) of mouse brain. The method provides sterolomic analysis at 400-µm spot diameter with a limit of quantification of 0.01 ng/mm2 It overcomes the limitations o

SUBMITTER: Yutuc E 

PROVIDER: S-EPMC7084107 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature