Mechanophenotyping of 3D multicellular clusters using displacement arrays of rendered tractions.

ABSTRACT: Epithelial tissues mechanically deform the surrounding extracellular matrix during embryonic development, wound repair, and tumor invasion. Ex vivo measurements of such multicellular tractions within three-dimensional (3D) biomaterials could elucidate collective dissemination during disease progression and enable preclinical testing of targeted antimigration therapies. However, past 3D traction measurements have been low throughput due to the challenges of imaging and analyzing information-rich 3D material deformations. Here, we demonstrate a method to profile multicellular clusters in a 96-well-plate format based on spatially heterogeneous contractile, protrusive, and circumferential tractions. As a case study, we profile multicellular clusters across varying states of the epithelial-mese

SUBMITTER: Leggett SE 

PROVIDER: S-EPMC7084145 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature