Sulfonamide Inhibition Studies of an α-Carbonic Anhydrase from Schistosoma mansoni, a Platyhelminth Parasite Responsible for Schistosomiasis.

ABSTRACT: Schistosomiasis is a debilitating infection provoked by parasitic flatworms called schistosomes. The species Schistosoma mansoni is endemic in Africa, where it causes intestinal schistosomiasis. Recently, an α-carbonic anhydrase (CA, EC 4.2.1.1) was cloned and characterized from this organism and designated as SmCA. The protein is expressed in the tegument (skin) of S. mansoni at the host-parasite interface. Recombinant SmCA possesses high catalytic activity in the CO₂ hydration reaction, similar to that of human CA isoform II with a k_cat of 1.2 × 10⁶ s^-1 and a k_cat/K_M of 1.3 × 10⁸ M^-1·s^-1. It has been found that schistosomes whose SmCA gene is suppressed using RNA interf

SUBMITTER: Angeli A 

PROVIDER: S-EPMC7084386 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature