Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality.

ABSTRACT: Genetic markers have been used to assess the freezability of semen. With the advancement in molecular genetic techniques, it is possible to assess the relationships between sperm functions and gene polymorphisms. In this study, variant calling analysis of RNA-Seq datasets was used to identify single nucleotide polymorphisms (SNPs) in boar spermatozoa and to explore the associations between SNPs and post-thaw semen quality. Assessment of post-thaw sperm quality characteristics showed that 21 boars were considered as having good semen freezability (GSF), while 19 boars were classified as having poor semen freezability (PSF). Variant calling demonstrated that most of the polymorphisms (67%) detected in boar spermatozoa were at the 3'-untranslated regions (3'-UTRs). Analysis of SNP abundance i

SUBMITTER: Mankowska A 

PROVIDER: S-EPMC7084667 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature