Synergistic Upregulation of Target Genes by TET1 and VP64 in the dCas9-SunTag Platform.

ABSTRACT: Overexpression of a gene of interest is a general approach used in both basic research and therapeutic applications. However, the conventional approach involving overexpression of exogenous genes has difficulty achieving complete genome coverage, and is also limited by the cloning capacity of viral vectors. Therefore, an alternative approach would be to drive the expression of an endogenous gene using an artificial transcriptional activator. Fusion proteins of dCas9 and a transcription activation domain, such as dCas9-VP64, are widely used for activation of endogenous genes. However, when using a single sgRNA, the activation range is low. Consequently, tiling of several sgRNAs is required for robust transcriptional activation. Here we describe the screening of factors that exhibit the best

SUBMITTER: Morita S 

PROVIDER: S-EPMC7084704 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature