Simple semi-high throughput determination of activity signatures of key antioxidant enzymes for physiological phenotyping.

ABSTRACT:

Background

Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide anions significantly accumulate during biotic and abiotic stress and cause oxidative damage and eventually cell death. There is accumulating evidence that ROS are also involved in regulating beneficial plant-microbe interactions, signal transduction and plant growth and development. Due to the relevance of ROS throughout the life cycle and for interaction with the multifactorial environment, the physiological phenotyping of the mechanisms controlling ROS homeostasis is of general importance.

Results

In this study, we have developed a robust and resource-efficient experimental platform that allows the determination of the activities of the nine key ROS scavenging enzymes from a single extraction

SUBMITTER: Fimognari L 

PROVIDER: S-EPMC7085164 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature