The phosphatidylethanolamine biosynthesis pathway provides a new target for cancer chemotherapy.

ABSTRACT:

Background & aims

Since human induced pluripotent stem cells (iPSCs) develop into hepatic organoids through stages that resemble human embryonic liver development, they can be used to study developmental processes and disease pathology. Therefore, we examined the early stages of hepatic organoid formation to identify key pathways affecting early liver development.

Methods

Single-cell RNA-sequencing and metabolomic analysis was performed on developing organoid cultures at the iPSC, hepatoblast (day 9) and mature organoid stage. The importance of the phosphatidylethanolamine biosynthesis pathway to early liver development was examined in developing organoid cultures using iPSC with a CRISPR-mediated gene knockout and an over the counter medication (meclizine) that inhibits the

SUBMITTER: Guan Y 

PROVIDER: S-EPMC7085447 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature