Lens aquaporins function as peroxiporins to facilitate membrane transport of hydrogen peroxide.

ABSTRACT: High levels of reactive oxygen species such as hydrogen peroxide (H₂O₂) cause oxidative stress in the lens and lead to cataractogenesis. The present investigation was undertaken to find out whether the mammalian lens aquaporins (AQPs) 0, 1, and 5 perform H₂O₂ transport across the plasma membrane to reduce oxidative stress. Our in vitro cell culture and ex vivo lens experiments demonstrated that in addition to the established water transport role, mouse AQP0, AQP1 and AQP5 facilitate transmembrane H₂O₂ transport and function as peroxiporins. Human lens epithelial cells expressing AQP1, AQP5 and AQP8, when treated with 50 μM HgCl₂ water channel inhibitor showed a significant reduction in H₂O₂ t

SUBMITTER: Varadaraj K 

PROVIDER: S-EPMC7085977 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature