Location and function of transient receptor potential canonical channel 1 in ventricular myocytes.

ABSTRACT: Transient receptor potential canonical 1 (TRPC1) protein is abundantly expressed in cardiomyocytes. While TRPC1 is supposed to be critically involved in cardiac hypertrophy, its physiological role in cardiomyocytes is poorly understood. We investigated the subcellular location of TRPC1 and its contribution to Ca²⁺ signaling in mammalian ventricular myocytes. Immunolabeling, three-dimensional scanning confocal microscopy and quantitative colocalization analysis revealed an abundant intracellular location of TRPC1 in neonatal rat ventricular myocytes (NRVMs) and adult rabbit ventricular myocytes. TRPC1 was colocalized with intracellular proteins including sarco/endoplasmic reticulum Ca²⁺ ATPase 2 in the sarcoplasmic reticulum (SR). Colocalization with wheat germ aggluti

SUBMITTER: Hu Q 

PROVIDER: S-EPMC7085981 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature