Quantification of ERK Kinase Activity in Biological Samples Using Differential Sensing.

ABSTRACT: The understanding of complex biological systems requires an ability to evaluate interacting networks of genes, proteins, and cellular reactions. Enabling technologies that support the rapid quantification of these networks will facilitate the development of biological models and help to identify treatment targets and to assess treatment plans. The biochemical process of protein phosphorylation, which underlies almost all aspects of cell signaling, is typically evaluated by immunoblotting procedures (Western blot) or more recently proteomics procedures, which provide qualitative estimates of the concentration of proteins and their modifications in cells. However, protein modifications are difficult to correlate with activity, and while immunoblotting and proteomics approaches have the poten

SUBMITTER: Zamora-Olivares D 

PROVIDER: S-EPMC7085986 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature