Identification of a selective manganese ionophore that enables nonlethal quantification of cellular manganese.

ABSTRACT: Available assays for measuring cellular manganese (Mn) levels require cell lysis, restricting longitudinal experiments and multiplexed outcome measures. Conducting a screen of small molecules known to alter cellular Mn levels, we report here that one of these chemicals induces rapid Mn efflux. We describe this activity and the development and implementation of an assay centered on this small molecule, named manganese-extracting small molecule (MESM). Using inductively-coupled plasma-MS, we validated that this assay, termed here "manganese-extracting small molecule estimation route" (MESMER), can accurately assess Mn in mammalian cells. Furthermore, we found evidence that MESM acts as a Mn-selective ionophore, and we obse

SUBMITTER: Horning KJ 

PROVIDER: S-EPMC7086026 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature