Osmotic and hypoxic induction of osteopontin in retinal pigment epithelial cells: Involvement of purinergic receptor signaling.

ABSTRACT:

Purpose

Osteopontin (OPN) is a neuroprotective factor in the retina that improves photoreceptor survival. The aim of the present study was to investigate whether human RPE cells express and respond to OPN.

Methods

Hypoxia and chemical hypoxia were induced by cell culture in 0.25% O₂ and the addition of CoCl₂, respectively. Hyperosmolarity was produced by the addition of 100 mM NaCl or 200 mM sucrose. Gene expression was quantified with real-time reverse transcription (RT)-PCR, and protein secretion was investigated with enzyme-linked immunosorbent assay (ELISA). Nuclear factor of activated T cell 5 (NFAT5) was depleted with siRNA.

Results

The acutely isolated RPE cells and the cultured RPE cells expressed OPN. OPN gene expression wa

SUBMITTER: Hollborn M 

PROVIDER: S-EPMC7086046 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature