Geometrical Constraints Greatly Hinder Formin mDia1 Activity.

ABSTRACT: Formins are one of the central players in the assembly of most actin networks in cells. The sensitivity of these processive molecular machines to mechanical tension is now well established. However, how the activity of formins is affected by geometrical constraints related to network architecture, such as filament cross-linking and formin spatial confinement, remains largely unknown. Combining microfluidics and micropatterning, we reconstituted in vitro mDia1 formin-elongated filament bundles induced by fascin, with different geometrical constraints on the formins, and measured the impact of these constraints on formin elongation rate and processivity. When filaments are not bundled, the anchoring details of formins have only a mild impact on their processivity and do not affect their elon

SUBMITTER: Suzuki EL 

PROVIDER: S-EPMC7086397 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature