High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers.

ABSTRACT:

Background

Skeletal muscles are composed of a heterogeneous collection of fiber types with different physiological adaption in response to a stimulus and disease-related conditions. Each fiber has a specific molecular expression of myosin heavy chain molecules (MyHC). So far, MyHCs are currently the best marker proteins for characterization of individual fiber types, and several proteome profiling studies have helped to dissect the molecular signature of whole muscles and individual fibers.

Methods

Herein, we describe a mass spectrometric workflow to measure skeletal muscle fiber type-specific proteomes. To bypass the limited quantities of protein in single fibers, we developed a Proteomics high-throughput fiber typing (ProFiT) approach enabling profiling of MyHC in single f

SUBMITTER: Kallabis S 

PROVIDER: S-EPMC7087369 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature