Silica nanomaterials induce organ injuries by Ca²⁺-ROS-initiated disruption of the endothelial barrier and triggering intravascular coagulation.

ABSTRACT:

Background

The growing use of silica nanoparticles (SiNPs) in many fields raises human toxicity concerns. We studied the toxicity of SiNP-20 (particle diameter 20 nm) and SiNP-100 (100 nm) and the underlying mechanisms with a focus on the endothelium both in vitro and in vivo.

Methods

The study was conducted in cultured human umbilical vein endothelial cells (HUVECs) and adult female Balb/c mice using several techniques.

Results

In vitro, both SiNP-20 and SiNP-100 decreased the viability and damaged the plasma membrane of cultured HUVECs. The nanoparticles also inhibited HUVECs migration and tube formation in a concentration-dependent manner. Both SiNPs induced significant calcium mobilization and generation of reactive oxygen species (ROS), increased the phosphorylat

SUBMITTER: Wang DP 

PROVIDER: S-EPMC7087393 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature