Long-term impact of a 4-day feed restriction at the protozoea stage on metabolic gene expressions of whiteleg shrimp (Litopenaeus vannamei).

ABSTRACT: Based on the "nutritional programming" concept, we evaluated the long-term effects of an early four-day caloric restriction (40% reduction in feed allowance compared to a normal feeding level) at the protozoea stage in whiteleg shrimp. We analyzed long-term programming of shrimp by studying metabolism at the molecular level, through RT-qPCR of key biomarkers (involved in intermediary metabolism and digestion). The mRNA levels (extracted from the whole body) were analyzed after the stimulus and after the rearing period, at 20 and 35 days, respectively. At the end of the experimental period, shrimp growth performance was evaluated. There was no difference between normal feed allowance (CTL) and feed-restricted shrimp (RES) for performance parameters (survival, final body weight and the numbe

SUBMITTER: Lage LPA 

PROVIDER: S-EPMC7087488 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature