Icariin affects cell cycle progression and proliferation of human retinal pigment epithelial cells via enhancing expression of H19.

ABSTRACT:

Background

Aberrant proliferation of retinal pigment epithelial (RPE) cells under pathologic condition results in the occurrence of proliferative vitreoretinopathy (PVR). Icariin (ICA)-a flavonol glucoside-has been shown to inhibit proliferation of many cell types, but the effect on RPE cells is unknown. This study aimed to clarify the inhibitory effects of ICA on RPE cells against platelet-derived growth factor (PDGF)-BB-induced cell proliferation, and discuss the regulatory function of H19 in RPE cells.

Methods

MTS assay was conducted to determine the effects of ICA on cell proliferation. Flow cytometry analysis was performed to detect cell cycle progression. Quantitative real-time PCR and western blot assay were used to measure the expression patterns of genes in RPE cell

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7087489 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature