A Primary Cell and Organoid Platform for Evaluating Pharmacological Responses in Mammary Epithelial Cells.

ABSTRACT: An essential process in predicting the in vivo pharmacological activity of a candidate molecule involves the evaluation of target responses using established model systems. While these models largely comprise immortalized cells, which are often serially passaged as monolayers on uniformly stiff substrates and are modified to overexpress one or more components of the pathway-of-interest, the importance of cell identity, heterogeneity, and three-dimensional (3D) context to target response is gaining increasing attention. Here, we assess intracellular calcium responses in mouse mammary epithelial cells in three distinct model systems: 3D primary organoids, 2D primary epithelial cells, and 2D immortalized cells. Specifically, we assess intracellular calcium responses to a number of extr

SUBMITTER: Stewart TA 

PROVIDER: S-EPMC7088941 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature