Characterization of basal and estrogen-regulated antisense transcription in breast cancer cells: Role in regulating sense transcription.

ABSTRACT: Estrogen-responsive breast cancer cells exhibit both basal and estrogen-regulated transcriptional programs, which lead to the transcription of many different transcription units (i.e., genes), including those that produce coding and non-coding sense (e.g., mRNA, lncRNA) and antisense (i.e., asRNA) transcripts. We have previously characterized the global basal and estrogen-regulated transcriptomes in estrogen receptor alpha (ERα)-positive MCF-7 breast cancer cells. Herein, we have mined genomic data to define three classes of antisense transcription in MCF-7 cells based on where their antisense transcription termination sites reside relative to their cognate sense mRNA and lncRNA genes. These three classes differ in their response to estrogen treatment, the enrichment of a number of genomic

SUBMITTER: Hou TY 

PROVIDER: S-EPMC7089808 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature