Effects of Housing Conditions and Circadian Time on Baseline c-Fos Immunoreactivity in C57BL/6J Mice.

ABSTRACT: Analysis of expression of the immediate early gene c-Fos in neuronal populations is a commonly used method to assess changes in neuronal activity due to various factors of interest. However, different levels of c-Fos have been observed between control animals across studies. The present investigation assessed whether such differences could reflect different behavioral or physiological states in housing conditions that are typically considered naïve controls. Specifically, we assessed c-Fos expression in 19 brain regions in male C57BL6/J mice that were housed either socially (in groups of four/cage) or individually. c-Fos expression was compared with socially-housed mice under either normal or reverse light conditions to assess the effect of light cycle on neuronal activity. We identified t

SUBMITTER: Robins MT 

PROVIDER: S-EPMC7089821 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature