High-throughput screening of environmental polysaccharide-degrading bacteria using biomass containment and complex insoluble substrates.

ABSTRACT: Carbohydrate degradation by microbes plays an important role in global nutrient cycling, human nutrition, and biotechnological applications. Studies that focus on the degradation of complex recalcitrant polysaccharides are challenging because of the insolubility of these substrates as found in their natural contexts. Specifically, current methods to examine carbohydrate-based biomass degradation using bacterial strains or purified enzymes are not compatible with high-throughput screening using complex insoluble materials. In this report, we developed a small 3D printed filter device that fits inside a microplate well that allows for the free movement of bacterial cells, media, and enzymes while containing insoluble biomass. These devices do not interfere with standard microplate readers an

SUBMITTER: Monge EC 

PROVIDER: S-EPMC7089899 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature