An Integrated In Vitro-In Silico Approach for Silver Nanoparticle Dosimetry in Cell Cultures.

ABSTRACT: Potential human and environmental hazards resulting from the exposure of living organisms to silver nanoparticles (Ag NPs) have been the subject of intensive discussion in the last decade. Despite the growing use of Ag NPs in biomedical applications, a quantification of the toxic effects as a function of the total silver mass reaching cells (namely, target cell dose) is still needed. To provide a more accurate dose-response analysis, we propose a novel integrated approach combining well-established computational and experimental methodologies. We first used a particokinetic model (ISD3) for providing experimental validation of computed Ag NP sedimentation in static-cuvette experiments. After validation, ISD3 was employed to predict the total mass of silver reaching human endothelial cells

SUBMITTER: Poli D 

PROVIDER: S-EPMC7089903 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature