The Costs of Close Contacts: Visualizing the Energy Landscape of Cell Contacts at the Nanoscale.

ABSTRACT: Cell-cell contacts often underpin signaling between cells. For immunology, the binding of a T cell receptor to an antigen-presenting pMHC initiates downstream signaling and an immune response. Although this contact is mediated by proteins on both cells creating interfaces with gap sizes typically around 14 nm, many, often contradictory observations have been made regarding the influence of the contact on parameters such as the binding kinetics, spatial distribution, and diffusion of signaling proteins within the contact. Understanding the basic physical constraints on probes inside this crowded environment will help inform studies on binding kinetics and dynamics of signaling of relevant proteins in the synapse. By tracking quantum dots of different dimensions for extended periods of time,

SUBMITTER: Kulenkampff K 

PROVIDER: S-EPMC7091464 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature