Promoter tools for further development of Aspergillus oryzae as a platform for fungal secondary metabolite production.

ABSTRACT:

Background

The filamentous fungus Aspergillus oryzae is widely used for secondary metabolite production by heterologous expression; thus, a wide variety of promoter tools is necessary to broaden the application of this species. Here we built a procedure to survey A. flavus genes constitutively highly expressed in 83 transcriptome datasets obtained under various conditions affecting secondary metabolite production, to find promoters useful for heterologous expression of genes in A. oryzae.

Results

To test the ability of the promoters of the top 6 genes to induce production of a fungal secondary metabolite, ustiloxin B, we inserted the promoters before the start codon of ustR, which encodes the transcription factor of the gene cluster responsible fo

SUBMITTER: Umemura M 

PROVIDER: S-EPMC7092444 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature