Identification of microRNAs and their Endonucleolytic Cleavaged target mRNAs in colorectal cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) ranks the third among the most common malignancies globally. It is well known that microRNAs (miRNAs) play vital roles in destabilizing mRNAs and repressing their translations in this disease. However, the mechanism of miRNA-induced mRNA cleavage remains to be investigated.

Method

In this study, high-throughput small RNA (sRNA) sequencing was utilized to identify and profile miRNAs from six pairs of colorectal cancer tissues (CTs) and adjacent tissues (CNs). Degradome sequencing (DS) was employed to detect the cleaved target genes. The Database for Annotation, Visualization and Integrated Discovery (DAVID) software was used for GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis.

Results

In total, 1

SUBMITTER: Zhou F 

PROVIDER: S-EPMC7092451 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature