Identifying nucleic acid-associated proteins in Mycobacterium smegmatis by mass spectrometry-based proteomics.

ABSTRACT:

Background

Transcriptional responses required to maintain cellular homeostasis or to adapt to environmental stress, is in part mediated by several nucleic-acid associated proteins. In this study, we sought to establish an affinity purification-mass spectrometry (AP-MS) approach that would enable the collective identification of nucleic acid-associated proteins in mycobacteria. We hypothesized that targeting the RNA polymerase complex through affinity purification would allow for the identification of RNA- and DNA-associated proteins that not only maintain the bacterial chromosome but also enable transcription and translation.

Results

AP-MS analysis of the RNA polymerase β-subunit cross-linked to nucleic acids identified 275 putative nucleic acid-associated proteins in the mo

SUBMITTER: Kriel NL 

PROVIDER: S-EPMC7092591 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature