Eya2 promotes cell cycle progression by regulating DNA damage response during vertebrate limb regeneration.

ABSTRACT: How salamanders accomplish progenitor cell proliferation while faithfully maintaining genomic integrity and regenerative potential remains elusive. Here we found an innate DNA damage response mechanism that is evident during blastema proliferation (early- to late-bud) and studied its role during tissue regeneration by ablating the function of one of its components, Eyes absent 2. In eya2 mutant axolotls, we found that DNA damage signaling through the H2AX histone variant was deregulated, especially within the proliferating progenitors during limb regeneration. Ultimately, cell cycle progression was impaired at the G1/S and G2/M transitions and regeneration rate was reduced. Similar data were acquired using acute pharmacological inhibition of the Eya2 phosphatase activity and the DNA

SUBMITTER: Sousounis K 

PROVIDER: S-EPMC7093111 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature