Absence of MyD88 from Skeletal Muscle Protects Female Mice from Inactivity-Induced Adiposity and Insulin Resistance.

ABSTRACT:

Objective

Inactivity and inflammation are linked to obesity and insulin resistance. It was hypothesized that MyD88 (mediates inflammation) knockout from muscle (MusMyD88^-/- ) would prevent, whereas miR146a^-/- (MyD88 inhibitor) would exacerbate, inactivity-induced metabolic disturbances.

Methods

Cre-control, MusMyD88^-/- , and miR146a^-/- mice were given running wheels for 5 weeks to model an active phenotype. Afterward, half were placed into a small mouse cage (SMC) to restrict movement for 8 days. Body composition, muscle (³ H)2-deoxyglucose uptake, visceral fat histology, and tissue weight (hind limb muscles, visceral fat, and liver) were assessed. In skeletal muscle and visceral fat, RNA sequencing and mitochondrial fun

SUBMITTER: Mahmassani ZS 

PROVIDER: S-EPMC7093260 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature