Time-resolved fluorescence microscopy with phasor analysis for visualizing multicomponent topical drug distribution within human skin.

ABSTRACT: Understanding a drug candidate's pharmacokinetic (PK) parameters is a challenging but essential aspect of drug development. Investigating the penetration and distribution of a topical drug's active pharmaceutical ingredient (API) allows for evaluating drug delivery and efficacy, which is necessary to ensure drug viability. A topical gel (BPX-05) was recently developed to treat moderate to severe acne vulgaris by directly delivering the combination of the topical antibiotic minocycline and the retinoid tazarotene to the pilosebaceous unit of the dermis. In order to evaluate the uptake of APIs within human facial skin and confirm accurate drug delivery, a selective visualization method to monitor and quantify local drug distributions within the skin was developed. This approach uses fluoresc

SUBMITTER: Jeong S 

PROVIDER: S-EPMC7093415 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature